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黄浦, 肖瑜, 覃妍, 梁美娜, 唐沈, 易茗, 朱芸
桂林理工大学
摘要:
以茶皂素为研究对象,以茶皂素的降解率为评价指标,利用地衣芽孢杆菌与铜绿假单胞菌混合发酵降解茶皂素,分别探讨接种比例、发酵时间、发酵温度、摇床转速及接种量对其降解的影响,再通过曲面响应设计实验对发酵条件进行优化。结果表明,影响降解率显著性次序为:发酵时间>发酵温度>接种量>摇床转速>接种比例,得到最佳的优化条件为:混菌接种比例1:2(v/v),发酵时间12.8d,发酵温度30.68℃,摇床转速150r/min,接种量为8.81%,在此条件下进行发酵,茶皂素的降解率达到(73.76±0.63)%。说明混菌对茶皂素的降解有着较明显的效果。
关键词:  地衣芽孢杆菌,铜绿假单胞菌,混菌发酵,茶皂素,条件优化
DOI:
分类号:X50
基金项目:
Optimum Conditions for Mixed Bacteria Degradation on Tea Saponin
HUANG Pu, XIAO Yu, QIN Yan, LIANG Meina, TANG Shen, YI Ming, ZHU Yun
Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology,Guilin University of Technology
Abstract:
Using tea saponin as raw material, with tea saponin degradation rate as the evaluation index,tea saponin was degraded by Bacillus licheniformis and Pseudomonas aeruginosa mixed, fermentation time, fermentation temperature, rotation speed and inoculum size on its degradation were investigated, and through the response surface design experiments the fermentation conditions were optimized. The results show that, the degradation rate was affected in the order as fermentation time > fermentation temperature > quantity of fermentation > rotation speed > vaccination ratio. The optimum fermentation degradation conditions were studied as mixed bacteria inoculation ratio of 1:2 (v/v), fermentation time 12.8 d, fermentation temperature 30.68 ℃, rotation speed 150 r/min, inoculated quantity 8.81%, in this condition for fermentation, decomposition rate of tea saponin reached (73.76 ± 0.63) %, indicating the mixed bacteria has obvious effect on the degradation of tea saponin.
Key words:  Bacillus licheniformis  Pseudomonas aeruginosa  mixed bacterium fermentation  tea saponin  conditions optimized

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