| 摘要: |
| 为避免牙膏中其他成分干扰检测，通过阳离子交换柱对牙膏溶液进行预处理，分离干扰物；再把氨甲环酸洗脱下来，合并浓缩后用HPLC进行检测。结果表明，牙膏空白基质不影响氨甲环酸的含量测定。氨甲环酸在50~250 mg/L 峰面积和浓度呈良好的线性关系，R2=0.999 8，平均回收率均大于99%，RSD均小于1%，最低检出限为3.128 mg/L，最低定量限为10.43 mg/L。可见，该方法准确、简便，专属性强，重现性好，可作为牙膏产品的质量控制方法。 |
| 关键词: 氨甲环酸 高效液相 牙膏 含量测定。 |
| DOI： |
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| HPLC Determination of Tranexamic Acid in Toothpaste |
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YE De-cheng1, PAN Qin2, ZHOU Kang1, CHEN Xiao-bin1
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1.Hawley Hazel Chemical Co Zhongshan Ltd,Guangdong;2.Zhongxin Innova Laboratories,Tianjin
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| Abstract: |
| To determine the content of tranexamic acid in toothpaste by HPLC. To avoid the interferences in toothpaste, separate the interference in toothpaste solution by cation exchange resin to get a purified sample. Detect the tranexamic acid amount by HPLC. Results show that the blank matrix has no effect on the determination of tranexamic acid. A good relationship was observed at 50~250 mg/L between peak area and concentration, with the correlation coefficient R2=0.9998. The average recoveries were more than 99% (RSDs ＜ 1.0%, n =3). The limit of detection (LOD) was 3.128 mg/L. The limit of quantitation (LOQ) was 10.43 mg/L. The method is easy, rapid, accurate, reproducible and highly specific. It can be used to control the toothpaste quality. |
| Key words: Tranexamic acid HPLC Toothpaste Content determination |
