| 摘要: |
| 建立了固相萃取与液相色谱质谱联用技术同时测定化妆品中7种局部麻醉药普鲁卡因、氯普鲁卡因、普鲁卡因胺、辛可卡因、利多卡因、丁卡因、苯佐卡因的方法。样品经体积分数为1%三氯乙酸-乙腈提取，固相萃取净化，液相色谱分离，选择特征离子多反应监测（ MRM）扫描模式测定，采用标准曲线法定量。结果表明，7种局部麻醉药在0.43~137 μg/L范围内均呈良好的线性关系，相关系数均大于0.99，检出限为0.86~1.38 μg/kg，加标回收率为80.1%~103.1%，相对标准偏差RSD小于5%（n =5)。 |
| 关键词: 化妆品 局麻药 固相萃取 液质联用 |
| DOI：10.13222/j.cnki.dc.2017.09.008 |
| 分类号: |
| 基金项目: |
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| Determination of seven local anesthetics in cosmetics by solid-phase extraction and liquid chromatography-mass spectrometry |
|
WANG Huan
|
|
Zhejiang Institute for Food and Drug Control
|
| Abstract: |
| A method was established for simultaneous determination of seven kinds of local anesthetics, i.e. procaine, chloroprocaine, procainamide, cinchocaine, lidocaine, tetracaine and benzocaine, in cosmetics by using solid-phase extraction and liquid
chromatography-mass spectrometry. The samples were extracted by 1% trichloroacetic acid-acetonitrile, purified by SPE and then isolated by liquid chromatography. Multi reaction monitoring (MRM) was used for quantitative analysis by standard curve method. It was found that the linearity for the seven drugs was good in the range from 0.43 to 137 μg/L, and the correlation coefficients were more than 0.99. The limit of detection was from 0.86 to 1.38 μg/kg. The recovery rate was in the range of 80.1%~103.1%, and the relative standard deviation (RSD) (n=5) was less than 5%. |
| Key words: cosmetics local anesthetics SPE LC-MS |
