| 摘要: |
| 利用乳酸菌发酵葡萄籽得到葡萄籽发酵液。对发酵液中的主要活性成分进行检测，通过测定自由基清除效果以及成纤维细胞增殖实验来衡量发酵液的抗衰老活性。结果表明葡萄籽发酵液中蛋白质、黄酮、原花青素的含量分别为2.89，1.01，0.43 mg/mL。体积分数为0.43%和1.25%的发酵液可清除50%的ABTS自由基和DPPH自由基；发酵液原液对羟自由基的清除率为40%，对铁离子的还原能力表示为2 073.43 µmol Trolox/L。 |
| 关键词: 抗衰老 葡萄籽 发酵液 活性成分 |
| DOI：10.13222/j.cnki.dc.2016.12.006 |
| 分类号:TQ658 |
| 基金项目:北京工商大学两科基金培育项目（LKJJ2016-09）；北京食品营养与人类健康高精尖中心专项经费；质检公益性行业科研专项项目（201310132,201410019） |
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| Study on anti-aging activity of grape seed fermentation liquor |
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XU Dan-ni1, SHI Dou-dou, ZHAO Dan, WANG Chang-tao2, LI Meng
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1.School of Science, Beijing Technology and Business University;2.Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University
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| Abstract: |
| The grape seeds were fermented by lactic acid bacteria to obtain fermentation liquor. Then the main active ingredients of
fermentation liquor were determined, and the anti-aging activity of the fermentation liquor was evaluated by measuring the free radicals
scavenging effect and the fibroblast proliferation. The results showed that the contents of protein, flavonoids and proanthocyanidins in
grape seed fermentation liquor were 2.89, 1.01 and 0.43 mg/mL, respectively. Fermentation liquor with volume fraction of 0.43% and
1.25% could scavenge 50% of ABTS and DPPH free radicals. The hydroxyl radicals scavenging rate and the ferric reducing ability of
fermentation liquor were 40% and 2 073.43 µmol Trolox/L, respectively. |
| Key words: anti-aging activity grape seed fermentation liquor active ingredients |
